TABLE 2.

Acyl-CoA synthetase enzyme activity

Enzyme	Substrate	Unacetylated activitya	Acetylated activity	Decrease
				%
PimA	Octanoate	6.9 ± 0.2	0.04 ± 0.02	99
MatB	Methyl malonate	8.6 ± 0.8	8.8 ± 1.01	0
BclM	Benzoate	6.2 ± 0.7	0.04 ± 0.03	99
P9 chimera	Methyl malonate	3.7 ± 0.2	0.05 ± 0.03	98
B1 chimera	Methyl malonate	6.1 ± 0.1	1.8 ± 0.32	71
B2 chimera	Methyl malonate	6.0 ± 0.2	2.1 ± 0.56	65
B3 chimera	Methyl malonate	6.8 ± 0.2	4.1 ± 0.39	40

^a Activity was reported as micromoles of AMP min⁻¹ mg⁻¹.