Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Removal of the GST tag with thrombin protease did not alter the ability of PX/PH domains to catalyze the exchange reaction. A, shown is SDS-PAGE of the purified proteins stained with Gel-code Blue showing GST-free PX, PH, and PXPH proteins at the expected molecular weights. Relative GDP dissociation (B) and GTP binding (C) of Rac2 in the presence of recombinant GST-free PX, PH, or PXPH (2 μm each) is shown. Rac2 alone (white) was used as a control.
