FIGURE 10.

d-Serine does not affect neuronal survival and neurite arborization. Cerebral cortex neurons were cultured for 12 and 3 days in control medium (Control; A and E) or in the presence of 0.4 mm d-serine (D-ser; B and F). Numbers of β-tubulin III cells (C), total cells (D), total neurite length (G and H), or neurites (I) were obtained using the ImageJ software. There was no statistical difference between the groups. Scale bars, 50 μm (A) and 30 μm (E). DIV, days in vitro. Error bars, S.E.