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TGF-β1 induces synaptogenesis in cerebral cortex neuronal cultures. Cerebral cortex neurons were cultured for 12 days in the presence of Neurobasal medium alone or supplemented with 10 ng/ml of TGF-β1. The number of synaptophysin/PSD-95 puncta was quantified after 12 days (A–C). Levels of synaptic proteins were evaluated by Western blotting assays (D). Scale bar, 20 μm. **, p < 0.0001. Error bars, S.E.
