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TGF-β1 does not affect neuronal survival and neurite arborization. Cerebral cortex neurons were cultured for 12 and 3 days in control medium (Control; A and E) or in the presence of 10 ng/ml TGF-β1 (TGF-β1; B and F). Numbers of β-tubulin III cells (C), total cells (D), total neurite length (G and H) or neurites (I) were obtained using the ImageJ software. There was no statistical difference between the groups. DIV, days in vitro. Scale bars, 50 μm (A) and 30 μm (E). Error bars, S.E.
