ATGL hydrolyzes TAGs with FAs of different chain length and saturation in vitro.
A, recombinant proteins were expressed in Cos-7 cells, and expression of His-tagged proteins was assessed by immunoblotting. B and C, homogenates of Cos-7 cells overexpressing ATGL were incubated in the absence or presence of CGI-58 with different, homogeneously (B) or heterogeneously esterified TAG species (C) as substrates for 1 h at 37 °C. Released FAs were measured using a NEFA-C kit. Data are normalized to LacZ and presented as the means ± S.D. and are representative of two independent experiments (***, p < 0.001; **, p < 0.01). O = oleic acid, C18:1; P = palmitic acid, C16:0.