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. 2012 Oct 12;287(49):41446–41457. doi: 10.1074/jbc.M112.400416

FIGURE 3.

FIGURE 3.

ATGL exhibits a preference for unsaturated FAs in vivo. A, WAT lipids of fed wt and ATGLko mice were extracted according to Folch et al. (21) and separated by TLC. TAGs were isolated and transesterified. FAMEs were separated and analyzed using GC/ flame ionization detector. B, plasma lipids of overnight-fasted wt and ATGLko mice were extracted and analyzed as in A. C, numeric changes in FA species of WAT TAGs and plasma FAs from wt and ATGLko mice are shown. D, plasma lipids of fed and overnight-fasted wt mice were extracted and analyzed as in A. E, numeric changes in plasma FA species of fed and fasted wt mice are shown. Data are presented as the means ± S.D. and are representative of two independent experiments (***, p < 0.001; **, p < 0.01; *, p < 0.05). n = 4 (each genotype).