FIGURE 6.

HSL hydrolyzes all isoforms of DAG. A, immunoblot analysis of lysates from Cos-7 cells expressing HSL or, as a control, LacZ using anti-His antibody is shown. B, lysate of Cos-7 cells expressing HSL were incubated with triolein or stereochemically different diolein substrates emulsified with phospholipids at 37 °C for 1 h. Generated FAs were measured using a NEFA-C kit (Wako Chemicals). Data are normalized to LacZ and presented as the means ± S.D. and are representative of two independent experiments (***, p < 0.001).