FIGURE 4.
Sirt1 or Sirt2 depletion prevents Ras-PI3K activation-induced decreases in H3K56ac. A, the efficiency of siRNA-mediated Sirt1 or Sirt2 knockdown was determined (left panel). The depletion of Sirt1 (middle panel) or Sirt2 (right panel) prevented the Ras-PI3K activation-induced decrease in the H3K56ac. HeLa cells were co-transfected as indicated. Whole cell lysates were assayed using Western blotting. B–D, HeLa cells were co-transfected with pEGFP-H-RasG12V/Y40C or pEGFP-N1 plasmids and a Sirt1- or Sirt2-specific or control siRNA as indicated (Ras, GFP, si-Sirt1, si-Sirt2, or si-con, respectively). As detected using Transwell and cell viability assays, Sirt1 or Sirt2 depletion resulted in reduced cell migration and cell viability (B and C). Cell cycle progression was measured using flow cytometry (D). E, HeLa cells were transfected with siRNA to deplete Sirt1 and Sirt2. The transcription of genes was examined using real time PCR. The values are presented as the means ± S.E. percentage relative to GAPDH expression.