Skip to main content
. 2012 Oct 11;287(49):41499–41513. doi: 10.1074/jbc.M112.384388

FIGURE 4.

FIGURE 4.

Gel filtration profiles of PfP2 from total parasite protein extracts from a synchronized P. falciparum culture at 18 and 30 h postmerozoite invasion. A and C, gel filtration profiles of about 3–4 mg of total parasite protein extracts in PBS from 18- and 30-h PMI parasite cultures, respectively, was resolved on a Superdex-200 gel filtration column and fractionated. Arrows indicates the elution positions of marker proteins. B and D, fractions eluted from A and C respectively, were run on 12% SDS-PAGE under reducing conditions and immunoblotted using anti-PfP2 monoclonal antibody (E2G12) (24). All fractions were tested, and only those fractions that showed reactivity with anti-PfP2 antibody (E2G12) are shown.