FIGURE 5.

Gel filtration profile of PfP2 from infected RBC cytosol (A and B) and RBC ghost (C and D) prepared from a synchronized P. falciparum culture at 30 h postmerozoite invasion. A and C, gel filtration profiles of 3–4 mg of protein from 30-h PMI P. falciparum-infected RBC cytosol and ghost, respectively, using Superdex-200 gel filtration column. Arrows indicate the elution positions of marker proteins. B and D, fractions eluted from A and C were run on 12% SDS-PAGE under reducing conditions and immunoblotted using anti-PfP2 monoclonal antibody (E2G12) (24). All fractions were tested, and only those fractions that showed reactivity with anti-PfP2 antibody (E2G12) are shown.