FIGURE 5.
Neuritin-induced IA densities and the increased expression of Kv4.2 α-subunit were dependent the Akt/mTOR signaling pathway. A, Western blot showing the levels of activated Akt (pAkt) and activated mTOR (p-mTOR) in CGNs incubated with 150 ng/ml neuritin for 15 min to 24 h. Chemiluminescent signals were detected with the ChemiDoc XRS System. B, effect of mTOR inhibitor, rapamycin, and Akt inhibitor LY294002 on neuritin-induced up-regulation of IA densities. C and D, effect of rapamycin and LY294002 on neuritin-induced up-regulation of Kv4.2 protein level. E, effect of rapamycin on neuritin-induced up-regulation of Kv4.2 mRNA expression measured by quantitative RT-PCR experiments. Data are shown as means ± S.E. (error bars). *, p < 0.05 compared with corresponding control by unpaired t test.