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. 2012 Oct 12;287(49):41534–41545. doi: 10.1074/jbc.M112.390260

FIGURE 7.

FIGURE 7.

Effect of neuritin on IR or IGF-1 receptor activation in CGNs and HeLa cells. A and B, immunoprecipitation assays showing that neuritin induced phosphorylation of IR, but not IGF-1 receptor in CGN lysate. Effect of insulin on phosphorylation of IGF-1 receptor is also shown. C, immunofluorescence to detect phosphorylated IR in HeLa cells. Effect of neuritin is also shown. Representative immunofluorescence images from four independent experiments are shown. Scale bar, 30 μm. D, immunohistochemical studies with DAB staining detecting the amount of phosphorylated IR in HeLa cells after neuritin stimulation. Effect of shRNA to reduce IR expression is also shown. Scale bar, 120 μm. Similar data were obtained from four independent experiments. E, immunofluorescence to detect phosphorylated IGF-1 receptor in HeLa cells. Effect of neuritin or insulin is also shown. Representative immunofluorescence image from four independent experiments are shown. Scale bar, 30 μm. F, immunohistochemical studies with DAB staining to detect phosphorylated IGF-1 receptor in HeLa cells. Effect of neuritin or insulin is also shown. Similar data were obtained from four independent experiments.