Figure 7. Spontaneous conformational transition of mPrP^wt after addition of disulfide-bond reducing agents. (A) The CD spectra of mPrP^wt (0.14 mg/mL) in 0.5 mM NaOAc, pH 7 with 1 mM DTT, recorded after incubated for the indicated time at 25°C. The protein was kept inside the quartz cuvette throughout the experiment. The direction of the spectral change is indicated by an arrow. (B) The CD spectrum of mPrP^wt (0.14 mg/mL) in 0.5 mM NaOAc, pH 7 with 1 mM TCEP was recorded immediately after sample preparation.