Figure 5.

Schematic representation of ds-oligonucleotides used in the investigation of protein binding in different sections of two promoter regions. Two series of ds-oligonucleotides were used to investigate protein binding in different sections of the pea atp9 (P1-P5) and the O. berteriana atp1 (O1-O5) promoter regions. Each ds-oligonucleotide represents a different section of one promoter region and was used as the DNA substrate in mobility-shift experiments (Fig. 6). The contiguous sequences of the two ds-oligonucleotide arrays correspond to the complete sequences of the atp9 and atp1 promoter regions from nucleotide position −79 to +56, respectively. Both promoter regions contain conserved nonanucleotide motifs (CNM) and AT-rich regions (AT-box). Numbering of the nucleotide positions refers to the transcriptional starting point (nucleotide position +1), represented by a bent arrow.