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. Author manuscript; available in PMC: 2012 Nov 30.
Published in final edited form as: Cytometry A. 2011 Nov 3;81(1):15–16. doi: 10.1002/cyto.a.21158

Figure 1.

Figure 1

An example of staining patterns and gating strategies for all fluorescent parameters. PBMC were stained according to the OMIP-004 protocol. (A) Debris and doublets were excluded from analysis. Live mononuclear cells were selected for sequential analysis. T cells were identified by CD3 expression. T cells subsets were identified by expression of CD4 and/or CD8. The CD4 subset was examined for expression of CD25 and Foxp3. (B) Representation of remaining parameters, in CD8+ T cells (left), Treg (center) and CD4+ T cells (right). (C) Expression of CCR7 and CD127 on naive CD8+ (left) and naive CD4+ T cells (right). Naive cells were gated as indicated in Figure 1A, through CD45RA and CD27 bivariate dot plots. Numbers in green next to the dot plots are a reference to the populations reported in online Supporting Information Table 6.