Table II.
Solubilization of the PM H+-ATPase and of the FC-FCBP complex from FC-PM with different detergents
| Protein:Detergent | PM H+-ATPase | FC-FCBP |
|---|---|---|
| mg mL−1:mg mL−1 | nmol Pi min−1 | pmol |
| Native PM | 387 (100%) | 33.75 (100%) |
| n-Octyl-β-d-glucopyranoside (1:8) | 185 (48%) | 16.87 (50%) |
| Chaps (1:16) | 205 (53%) | 22.50 (67%) |
| Dodecyl-β-d-maltoside (1:1) | 120 (31%) | 11.13 (33%) |
FC-PM protein (1 mg) was solubilized with the specified detergents, as described in Methods. PM H+-ATPase activity was assayed in the pellet at pH 6.4. FC-FCBP was measured in the pellet by FC RIA.