Table IV.
pH ratio of PM H+-ATPase activity in Suc-density gradient peak fractions from C-PM and FC-PM
| Fraction | PM H+-ATPase
Activity
|
pH Ratio
|
||||
|---|---|---|---|---|---|---|
| pH
6.4
|
pH 7.5
|
C-PM | FC-PM | |||
| C-PM | FC-PM | C-PM | FC-PM | |||
| nmol Pi min−1 | ||||||
| Dodecyl-β-d-maltoside SN | 1608 | 2202 | 324 | 1200 | 5 | 1.8 |
| Fraction 12 | 510 | 402 | 36 | 102 | 14.2 | 4 |
| Fraction 13 | 468 | 822 | 30 | 282 | 15.6 | 2.9 |
| Fraction 14 | 276 | 618 | 18 | 234 | 15.3 | 2.6 |
Assays were performed on the specified fractions of the gradient in Figure 2 in the presence of 10% (v/v) glycerol. pH ratio is the ratio between the activity measured at pH 6.4 and that measured at pH 7.5.