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Layout of plate for the screening of 80 peptide mixtures by competitive ELISA. A 96-well microtiter plate is coated with antigen and incubated with antibody and peptide mixtures to test for inhibitory activity by the mixtures. Peptide mixtures are tested in duplicate on a second plate; wells containing antibody only (− ; column 1) and antibody plus the soluble antigen of interest (+; column 2) are included in each row.
