Table 9.5.2.

Examples of Conditions Used in T Cell Activation Assays with Positional Scanning Libraries

Assay	Clone	No. of cells/well		APC:TCC RATIO	APC	Timea	Reference
		TCC	APC
Thymidine incorporation	Human/CD4+	2.5 × 105	1 × 105	4	PBMC	48 hr	Hemmer et al., 2000
	Human/CD4+	2 × 104	5 × 104	2.5	PBMC	48 hr	Hemmer et al., 1999
	Human/CD4+	2 × 104	1 × 105	5	PBMC	48 hr	Sospedra et al, 2006
	Human/CD4+	2 × 104	5 × 104	2.5	BLS	48 hr	Sospedra et al, 2006
	Human/CD4+	3 × 104	1 × 105	3.3	PBMC	72 hr	Venturini et al, 2006
	Mouse/CD4+b	3 × 105c	N/Ad	N/Ad	N/Ad	48 hr	Wilson et al., 1999; Judkowski et al., 2001
Chromium release	Human/CD8+	1 × 104	1 × 103	0.1	T2 line	4 hr	Pinilla et al., 2001
	Human/CD8+	1 × 104	1 × 103	0.1	T2 line	4 hr	Rubio-Godoy et al, 2002a
	Human/CD8+	1 × 104	1 × 103	0.1	T2 line	4 hr	Rubio-Godoy et al, 2002c
	Human/CD8+	2 × 103	1 × 104	5	T2 line	4 hr	Boggiano et al, 2005
Gamma interferon production	Mouse/CD8+	3 × 104	1 × 104	0.3	T2-A2.1/Kb	20 hr	Lustgarten et al, 2006
GM-CSF production	Human/CD4+	2.5 × 104	5 × 104	2	LCL	48 hr	Judkowski et al, 2011

^aTime of incubation with the mixtures from positional scanning library.

^bTCR-transgenic mice-CD4⁺.

^cWhole spleen cells.

^dNot applicable.