Table 1.
RNA melting temperatures (TM) at pH 5 and 7
| RNA with predicted or known ENE* | pH 7 |
pH 5 |
||
| TM,1† | TM,2 | TM,1 | TM,2 | |
| KSHV PAN ENE core + A32GA2 | 56 ± 1 | 76.8 ± 0.6 | 49.0 ± 0.7 | 77 ± 1 |
| MALAT1 ENE+A WT | 60.9 ± 0.2 | 76.6 ± 0.6 | 66.2 ± 0.3 | 75 ± 1 |
| MALAT1 ENE+A GC to AA | 45 ± 1 | 76.5 ± 0.4 | 46.7 ± 0.6 | 75.7 ± 0.3 |
| MENβ ENE+A WT | 63.3 ± 0.5 | 83.9 ± 0.7 | 69.0 ± 0.9 | 86.3 ± 0.3 |
| MENβ ENE+A GC to AA | 41.9 ± 0.5 | 83 ± 1 | 46.3 ± 0.4 | 85.6 ± 0.6 |
*Schematics of RNA structures are shown in Fig. 2E
†Melting temperatures are reported in degrees Celsius as the average of three independent measurements ± SD.