Fig. 4.
A1304T mutation alters hNav1.8 channel properties. (A) Representative hNav1.8 WT (black) and A1304T (red) currents recorded from DRG neurons. (B) Activation for A1304T channels was hyperpolarized by ∼6 mV compared with WT, whereas steady-state fast inactivation of WT and A1304T were not significantly different. (C) Averaged ramp currents (normalized to maximal transient peak current) produced by WT Nav1.8 (black; n = 11) and A1304T (red; n = 12) channels. SEM is indicated by error bars. Voltage dependence of ramp currents for A1304T was hyperpolarized by 6 mV compared with WT. (D) Mean normalized voltage of peak ramp current for A1304T channels (−28.5 ± 1.1 mV; n = 12; *P < 0.05) was significantly different from that for WT channels (−22.5 ± 1.9 mV; n = 11). Data are presented as means ± SEM. *P < 0.05.