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Glycosaminoglycans (GAGs) production. (A) Safranin-O/Fast-Green/Hematoxylin stained section from pellets of GFP+ or GFP− cells passaged once (Left), twice (Center), or three times (Right) after sorting. (Scale bar: 100 µm.) (B and C) Total GAGs per pellet (B) andGAGs per DNA by DMB assay (C), asterisk indicates P < 0.05 to GFP− of same passage and pound indicates P < 0.05 to passage 1 of own cell type, n ≥ 4 per group, mean ± SEM.
