Effect of DTT and nigericin on the time course of
qN in wheat leaf tissue. Detached leaves were supplied with 3
mm DTT (○, •) (A) or 100 μm nigericin
(○, •) (B) via the transpiration stream. Detached control leaves
(□, ▪) were allowed to transpire in water. Subsequently, leaf
tissue was incubated in the dark for 15 min at 25°C (○, □) or for
10 min at 25°C followed by 5 min at 37.5°C (•, ▪). After
incubation, the time course of Chl fluorescence was measured at 25°C.
Data points represent the mean ± se of two
replications.