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Effect of temperature on the form of phosphoribulokinase in the soluble leaf extracts of wheat (A) and cotton (B). Detached leaf tissue was incubated for the indicated times and at the indicated temperatures and then processed and analyzed as described in Figure 5. Lanes were loaded with extract corresponding to equal amounts of leaf area. Only the supernatant fraction is shown because there was no phosphoribulokinase visible on western blots of proteins from the pellet fraction.
