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RVFV N RNA-binding interactions. Amino acids and RNA nucleotides are rendered in stick form. The carbon atoms in amino acids from adjacent subunits are colored green and gray. The 2.15-Å electron density (2Fo–Fc, contoured at 1σ, unbiased density in Fig. S7) shows RNA bound to an N subunit in RVFV N4-RNA28. Amino acids labeled in red are invariant in phlebovirus N proteins. RNA bases 1–7 are labeled B1 to B7 and B1′ is B1 of the adjacent N subunit. Dashed lines represent hydrogen bonds.
