Skip to main content
. 2012 Nov 5;109(47):19226–19231. doi: 10.1073/pnas.1211491109

Fig. 3.

Fig. 3.

PTP1B regulates Tyr phosphorylation and the stability of IFNAR1 in human cells. (A) Endogenous IFNAR1 immunopurified from 293T cells that received indicated shRNA and were treated with IFN-α or IL-1β (as indicated) was analyzed by immunoblotting using the indicated antibodies. (B) Degradation of endogenous IFNAR1 in 293T cells transfected and treated as indicated was analyzed by immunoprecipitation–immunoblotting. Levels of actin in samples were also analyzed. (C) Primary sequence alignment of proximal fragments of the intracellular domains of IFNAR1 proteins from indicated species. Tyr-based endocytic motifs are depicted in bold. (D) MEFs from animals with indicated genotype were treated with PBS or murine IFN-β and CHX as indicated. Levels of murine IFNAR1 and actin were analyzed. (E) Internalization rate of chimeric mouse–human IFNAR1 proteins (chWT or chYF) coexpressed with PTP1B as indicated in MEFs from IFNAR1 knockout mice. (F) Turnover rate of FLAG-tagged mouse (mR1) or chimeric mouse–human IFNAR1 (chWT or chYF) expressed in WT or PTP1B-null MEFs treated with mIFN for indicated times.