Fig. 1.

Cripto is expressed during skeletal muscle regeneration. Double immunofluorescence with anti-laminin (green) and anti-Cripto (red) antibodies is illustrated in uninjured TA muscle (A–C) and in CTX-injured TA muscles during regeneration (D–F), showing Cripto expression in regenerating fibers (white arrows). (G–L) Regenerating muscle sections stained with anti-Cripto (red) and anti-MyoD or F4/80 (green) antibodies. Colocalization of Cripto and MyoD (green) or F4/80 (green) indicates Cripto expression in myogenic cells (G–I, white arrows) and inflammatory cells (J–L, yellow arrowheads), respectively. Nuclei are stained in blue with DAPI. (Scale bars = 50 μm.) See also Fig. S1.