Push-push negative feedback. (A) Circuit and templates. On injection of δ, production of β is activated. Then, β hybridizes to βtoiα (resulting in an increase of TAMRA fluorescence) and to βtoiδβ, which, in turn, produces the inhibitor of δtoβ, stopping the production of β. (B) Experimental time plot of the TAMRA fluorescence in normalized units (N.U.; 1 at the highest and 0 at the lowest) for different concentrations (0, 1, 2, and 4 nM) of inhibition module βtoiδβ.