Table 1.
Polypeptide spots that showed at least a 10-fold increase in relative abundance in B31 or N40D10/E9 on 2D protein gel
| Spot # | pI | MW (kDa) | Relative abundance in B31, and N40 (%) | Fold change B31 vs N40 | Identification MALDI-MS analyses (SwissProt or NCBI accession #) | Spot # | pI | MW (kDa) | Relative abundance in B31, and N40 (%) | Fold change N40 vs B31 | Identification MALDI-MS analyses (SwissProt or NCBI accession #) |
|---|---|---|---|---|---|---|---|---|---|---|---|
|
33 |
6.2 |
88.96 |
0.036, 0.003 |
11.2 |
|
136 |
5.6 |
64.58 |
0.002, 0.029 |
14.7 |
|
|
110 |
5.1 |
63.92 |
0.050, 0.003 |
15.1 |
|
208 |
5.8 |
53.07 |
0.015, 0.340 |
22.7 |
|
|
127 |
5.3 |
65.24 |
0.037, 0.003 |
11.5 |
|
231 |
6.9 |
52.81 |
0.019, 0.226 |
11.8 |
|
|
211 |
6.1 |
55,65 |
0.875, 0.048 |
18.0 |
|
272 |
6.2 |
46.29 |
0.000, 0.054 |
685.4 |
*Flagellin (GI:120230), Basic membrane protein A (GI:3913169) |
|
225 |
6.1 |
57.07 |
0.193, 0.005 |
35.3 |
|
293 |
6.0 |
43.53 |
0.000, 0.170 |
698.2 |
*Flagellin (GI:120230) |
|
325 |
5.6 |
38.32 |
0.114, 0.010 |
11.3 |
|
311 |
6.0 |
39.99 |
0.005, 0.165 |
30.6 |
|
|
403 |
5.4 |
31.03 |
0.071, 0.002 |
29.1 |
|
347 |
6.0 |
35.06 |
0.003, 0.185 |
59.8 |
|
|
404 |
5.4 |
31.00 |
0.404, 0.003 |
124.1 |
OspD (GI:495462) |
348 |
5.6 |
34.95 |
0.007, 0.258 |
36.3 |
|
|
405 |
5.5 |
28.78 |
1.006, 0.031 |
32.7 |
|
349 |
6.0 |
34.36 |
0.003, 0.095 |
32.4 |
|
|
458 |
5.7 |
26.07 |
0.051, 0.003 |
15.2 |
|
352 |
6.5 |
34.25 |
0.003, 0.034 |
12.0 |
|
|
463 |
6.5 |
25.58 |
0.107, 0.007 |
15.4 |
|
354 |
5.9 |
34.62 |
0.004, 0.207 |
47.9 |
|
|
465 |
6.3 |
25.49 |
0.077, 0.006 |
13.2 |
|
381 |
5.6 |
29.61 |
0.003, 0.032 |
10.4 |
|
|
491 |
7.0 |
22.69 |
0.356, 0.012 |
29.8 |
|
406 |
6.2 |
28.54 |
0.006, 0.081 |
14.0 |
|
|
494 |
6.5 |
23.16 |
1.400, 0.062 |
22.8 |
|
418 |
6.3 |
27.97 |
0.089, 1.927 |
21.6 |
|
|
495 |
6.7 |
23.13 |
6.875, 0.025 |
278.1 |
Outer surface protein C (GI:3914248) |
452 |
6.6 |
26.33 |
0.006, 0.246 |
42.5 |
30S ribosomal protein S4 (B7J2H5) Phosphoglycolate phosphatase (GI:226320487), and hypothetical (GI:226315606) |
|
497 |
6.3 |
22.87 |
0.262, 0.022 |
12.1 |
|
479 |
6.4 |
24.51 |
0.060, 0.648 |
10.7 |
|
|
519 |
7.1 |
20.08 |
0.734, 0.027 |
26.8 |
|
501 |
6.5 |
22.47 |
0.030, 1.956 |
64.6 |
Same as 505 |
|
525 |
6.4 |
21.03 |
0.234, 0.008 |
30.9 |
Neutrophil activating protein (GI:15595035) |
505 |
6.3 |
22.33 |
0.017, 0.570 |
34.0 |
OspC (GI: 226246807) Neutrophil activating protein (GI:15595035) |
|
528 |
6.2 |
20.95 |
0.068, 0.004 |
15.9 |
|
517 |
6.2 |
21.41 |
0.002, 0.095 |
54.4 |
|
|
541 |
6.4 |
20.31 |
0.097, 0.005 |
20.8 |
|
543 |
5.6 |
19.67 |
0.006, 0.072 |
11.9 |
|
|
559 |
5.6 |
17.70 |
0.137, 0.008 |
17.2 |
|
551 |
6.2 |
19.51 |
0.075, 0.762 |
10.2 |
|
|
581 |
4.9 |
11.91 |
2.069, 0.048 |
42.7 |
6.6 kDa lipoprotein (GI:1477781) |
573 |
5.3 |
14.07 |
0.005, 0.255 |
55.0 |
|
|
585 |
6.3 |
28.02 |
0.125, 0.010 |
12.2 |
|
|
|
|
|
|
|
|
586 |
6.1 |
44.19 |
0.357, 0.001 |
674.8 |
*Flagellin (GI:120230) |
|
|
|
|
|
|
|
587 |
6.1 |
44.41 |
0.209, 0.000 |
765.7 |
|
|
|
|
|
|
|
| 588 | 6.1 | 41.54 | 0.276, 0.001 | 527.4 |
* Flagellin appears to be produced at equivalent levels in both strains but fold change depicted is higher for respective spots due to slight mobility differences of this protein in B31 and N40D10/E9 gels.