Figure 1. A neighbor-joining tree of isolates based on the D1/2 region unique sequences.

The evolutionary distance (refer to the bar) was calculated according to Kimura [26]. Bootstrap values [27] were summarized by black dots (indicating at least 90% support) or by black rings (indicating at least 60% support). Blue solid ring, new species candidate isolated from a plant of Iriomote Island; blue open ring, described species isolated from a plant of Iriomote; blue solid square, new species candidate isolated from soil of Iriomote; blue open square, described species isolated from soil of Iriomote; blue solid ring, new species candidate isolated from a plant of Rishiri Island; red open ring, described species isolated from a plant of Rishiri; red solid square, new species candidate isolated from soil of Rishiri; red open square, described species isolated from soil of Rishiri. Numerals in brackets after ring or square indicate the number of isolates. Yellow color on OTUs indicates this species was isolated from both islands.