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The numbers of INF-γ secreting splenic CD8+T cells in vaccinated mice. Mice were immunized as described in method section. 3 weeks after the last immunization, the number of IFN-γ secreting CD8+ T cells in the spleen from the different groups of mice was evaluated by ELISpot. The results represent the averages of triplicate wells of three mice, and are expressed as means ± SD, * Significant difference (P<0.05).
