Table 1. FACS analysis of the B cell populations present in the bone marrow three months after competitive bone marrow transplantation.

Donor	1∶1	WT:WT		1∶1	WT:KO
% cells	gated	CD45.1+	CD45.2+	gated	CD45.1+	CD45.2+
FSC-A/SSC-A	24.1	29.5	37.4	28.2	23.9	46.4***
B220+	53.9	39.9	56.1	58.5	30.6*	66.0*
AA4.1+	47.6	37.9	57.7	51.3	26.1	70.6
IgM-CD23+	1.3	48.7	47.1	2.2	28.7*	66.9*
IgM+CD23+	6.3	46.1	47.3	7.1	30.7*	62.9*
IgM+CD23−	16.9	38.4	57.6	18.4	25.6	70.8
IgM-CD23−	75.4	36.9	56.8	72.3	25.8	69.2
FSC-A/SSC-A	61	31.1	50.3	55.0	22.9*	56.4
B220-	72.3	23.7	39.2	66.1	17.2	39.6
B220+IgM+	23.5	42.3	54.9	24.8	29.4	62.7
B220++IgM+	76.1	42.3	54.9	74.2	33.6*	63.1*
B220+IgM−	10.8	42.7	48.0	12.8	27.0*	57
Pre B	11.2	46.4	49.4	10.4	13.4***	53.7
Pro B	82.0	42.3	50.0	84.8	28.4	56.4

Distribution of CD45.1+ and CD45.2+ cells in the bone marrow of recipient mice 3 months after bone marrow transplantation. CD45.1+ WT and CD45.2+ WT or CD45.2+ KO donor cells were mixed at a 1∶1 ratio prior to injection into recipient CD45.1+ mice. The percentage of total cells gated and the percentage of CD45.1+ or CD45.2+ cells for any given gate are shown. Significance was assessed using Student’s t-test and p-values are indicated as. *p<0.05, **p<0.01, ***p<0.001.