Table 2. FACS analysis of the cellular populations present in the spleen three months after competitive bone marrow transplantation.

Donor	1∶1	WT:WT		1∶1	WT:KO
% cells	gated	CD45.1+	CD45.2+	gated	CD45.1+	CD45.2+
FSC-A/SSC-A	82.5	39.7	58.2	82.5	37.1	61.0
CD19+	68.5	35.2	62.9	66.4	33.7	64.3
Fo	81.9	35.4	63.8	81.1	35.1	64.0
MZ	6.2	44.7	52.9	5.5	35.14*	62.54**
CD1d+/T1	3.4	23.1	60.9	3.8	19.4	65.3
Cd1d+/T2-Fo	91.1	35.5	63.5	89.8	34.7	63.9
CD19+CD1d++	4.2	40.3	56.3	4.0	33.16*	63.86*
MZ	61.7	44.4	52.8	62.2	33.24***	64.38***
Pre-MZ	9.6	33.5	59.1	6.6	37.7	54.5
FSC-A/SSC-A	81.7	39.9	58.6	80.34	37.52	61.1
B220+	69.3	35.6	63.1	67.3	34.0	64.6
AA4.1+	13	34.7	62.3	14	25.3*	71.9*
IgM-CD23+	42.6	38.7	59.5	40.8	28.2*	70.2*
IgM+CD23+	26.3	34.6	62.4	25.3	24.1**	73.3**
IgM+CD23−	18.6	29.9	65.3	20.0	20.8**	73.8*
IgM−CD23−	12.5	28.8	66.7	14	24.7	72.2
FSC−A/SSC-A	82.6	40.1	58.5	82.3	37.4	61.1
CD19−	30.4	50.4	47.4	34.1	43.7	54.4
CD8−CD4+	56.9	50.6	49.3	59.9	46	53.8
CD8+CD4+	0.2	47.6	45.1	0.2	42.5	47
CD8+CD4−	29.1	53.7	45.8	25.7*	46.5	53.1
CD8−CD4−	13.9	42.2	43.7	14.2	29.8*	58.6*
FSC-A/SSC-A	91.8	35.9	58.5	91.8	34.1	61.4
CD11c+	4.4	35.7	51.4	4.6	26.0*	62.2*
Gr1+	15.4	51.0	33.9	13.3	42.9	43.7
Mac1+	17.0	39.8	39.1	15.8	27.6	53.9

Distribution of CD45.1+ and CD45.2+ cells in the spleen of recipient mice 3 months after bone marrow transplantation. CD45.1+ WT and CD45.2+ WT or CD45.2+ KO donor cells were mixed at a 1∶1 ratio prior to injection into recipient CD45.1+ mice. The percentage of total cells gated and the percentage of CD45.1+ or CD45.2+ cells for any given gate are shown. Significance was assessed using Student’s t-test and p-values are indicated as *p<0.05, **p<0.01, ***p<0.001.