Table 3. FACS analysis of the cellular populations present in the thymus three months after competitive bone marrow transplantation.

Donor	1∶1	WT:WT		1∶1	WT:KO
% cells	gated	CD45.1+	CD45.2+	gated	CD45.1+	CD45.2+
FSC-A/SSC-A	95.5	35.7	63.6	95.5	19.4**	79.9**
CD19+	0.4	38.1	56.5	0.5	33.7	58.3
CD19−	99.5	35.8	63.7	99.5	19.4**	80.0**
CD8−CD4+	9.2	39.4	60.5	10.9	21.4**	78.4**
CD8+CD4+	86.3	35.4	64.3	84.2	19.0**	80.5**
CD8+CD4−	2.6	41.8	57.8	3.2	22.4**	77*
CD8−CD4−	1.9	19.4	45.9	1.7	17.4	69.3

Distribution of CD45.1+ and CD45.2+ cells in the thymus of recipient mice 3 months after bone marrow transplantation. CD45.1+ WT and CD45.2+ WT or CD45.2+ KO donor cells were mixed at a 1∶1 ratio prior to injection into recipient CD45.1+ mice. The percentage of total cells gated and the percentage of CD45.1+ or CD45.2+ cells for any given gate are shown. Significance was assessed using Student’s t-test and p-values are indicated as *p<0.05, **p<0.01, ***p<0.001.