Table 4. FACS analysis of the B cell populations present in the peritoneal cavity three months after competitive bone marrow transplantation.

Donor	1∶1	WT:WT		1∶1	WT:KO
% cells	gated	CD45.1+	CD45.2+	gated	CD45.1+	CD45.2+
FSC-A/SSC-A	56.2	46.4	52.4	43.1	41.5	53.5
B1	11.1	50.1	48.6	9.2	63.4**	34.8**
CD5+	57.2	47.3	51.3	52.4	60.9*	37.5*
CD5−	41.4	53.2	45.9	46.2	66.1***	32.1***
B2	57.2	42.1	56.7	53.2	38.8*	59.9
CD5+	16.6	38.7	58.2	13.7	38.2	58.0
CD5−	81.0	42.9	56.4	83.3	39.0*	60.1

Distribution of CD45.1+ and CD45.2+ cells in the peritoneal cavity of recipient mice 3 months after bone marrow transplantation. CD45.1+ WT and CD45.2+ WT or CD45.2+ KO donor cells were mixed at a 1∶1 ratio prior to injection into recipient CD45.1+ mice. The percentage of total cells gated and the percentage of CD45.1+ or CD45.2+ cells for any given gate are shown. Significance was assessed using Student’s t-test and p-values are indicated as *p<0.05, **p<0.01, ***p<0.001.