Table 6. FACS analysis of the B cell populations present in the Peyer’s patches three months after competitive bone marrow transplantation.
Donor | 1∶1 | WT:WT | 1∶1 | WT:KO | ||
% cells | gated | CD45.1+ | CD45.2+ | gated | CD45.1+ | CD45.2+ |
Ungated cells | 20.1 | 32.3 | 24.4 | 33.5 | ||
CD19+ | 45.3 | 28.5 | 53.6 | 46.5 | 34.9 | 50.7 |
CD38+ | 68.8 | 32.9 | 63.0 | 72.0 | 38.8 | 57.1* |
FAS+ | 5.2 | 50.0 | 38.3 | 5.3 | 50.5 | 40.1 |
GC | 22.9 | 10.6 | 25.5 | 19.7 | 12.9 | 28.0 |
Distribution of CD45.1+ and CD45.2+ cells in the Peyer’s patches of recipient mice 3 months after bone marrow transplantation. CD45.1+ WT and CD45.2+ WT or CD45.2+ KO donor cells were mixed at a 1∶1 ratio prior to injection into recipient CD45.1+ mice. The percentage of total cells gated and the percentage of CD45.1+ or CD45.2+ cells for any given gate are shown. Significance was assessed using Student’s t-test and p-values are indicated as *p<0.05, **p<0.01, ***p<0.001.