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. 2012 Nov 30;7(11):e50699. doi: 10.1371/journal.pone.0050699

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© 2012 Hofreuter et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) The serine utilization locus of C. jejuni consists of the sdaCA operon, which is conserved in all sequenced C. jejuni strains. The genes sdaC and sdaA encode for a serine uptake transporter and a L-serine dehydratase, respectively. The intergenic sequence is shown with the sdaA start codon (ATG), the sdaA shine-dalgarno squence (underlined) and the sdaC stop codon (*). Mutants of sdaA and sdaC were constructed by insertion of kanamycin and erythromycin resistance cassettes in respective genes as indicated. (B) Comparison of different C. jejuni wild-type isolates to utilize serine as growth substrate. The shown optical densities are the mean values ±SD that were maximally reached by indicated wild-type C. jejuni strains RM1221, 81116, 305, 327 and DFVF1099 during a growth period of 48 hours in DMEM supplemented with 20 mM of the indicated amino acids.