Figure 1. dCIA30 mutation results in loss of complex I holoenzyme.

(A) dCIA30 transcript levels were measured by qRT-PCR in male third instar (L3) larvae (n = 5 male L3 larvae, 3 replicates). The dCIA30^EY09101 and dCIA30^ex80 insertion mutants show a ca. 55–75% loss of transcript relative to a precise excision control (* p<0.05). (B) The dCIA30^EY09101 and dCIA30^ex80 mutations result in a specific loss of the band that corresponds to complex I holoenzyme in blue native polyacrylamide gel electrophoresis (BN-PAGE) of L3 larvae. Precise excision controls (+) and larvae with expression of a dCIA30 cDNA construct (UAS-dCIA30/+; da-GAL4, dCIA30^ex80/dCIA30^ex80, “cDNA rescue”) show the presence of the complex I holoenzyme band. (M = molecular size marker, CV2 = complex V dimer, CI = complex I, CV1 = complex V monomer, CIII = complex III, CIV = complex IV, CII = complex II, mitochondria from 2.5, 5, and 10 larvae equivalents in successive lanes for each genotype).