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(A) Activation of apo-NHase and apo-NHase(HisT7-α) by holo-α(StrepP14K)2. Apo-NHase or apo-NHase(HisT7-α) (final concentration, 0.1 mg/ml) was incubated with the purified holo-α(StrepP14K)2 (final concentration, 0.8 mg/ml) in 10 mM KPB (pH 7.5) at 24°C. (B) SDS-PAGE of apo-NHase(HisT7-α) and R-apo-NHase(HisT7-α). 1, markers; 2, apo-NHase(HisT7-α); 3, purified R-apo-NHase(HisT7-α).
