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. 2012 Nov 30;7(11):e50829. doi: 10.1371/journal.pone.0050829

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© 2012 Liu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Expression and purification of NHases encoded by the A-B-P14K and B-A-P14K genes. 1, markers; 2, cell extract of the transformant containing pET-A-B-P14K; 3, cell extract of the transformant containing pET-B-A-P14K; 4, purified NHase from the transformant containing pET-A-B-P14K; 5, purified NHase from the transformant containing pET-B-A-P14K. (B) Catalytic efficiency of the two NHases encoded by B-A-P14K was a little superior to that encoded by A-B-P14K. The two transformants harboring pET-A-B-P14K and pET-B-A-P14K were incubated under the same condition as described in EXPERIMENTAL PROCEDURES, respectively, high concentration of 3-cyanopyridine (430 mM, final) was added after IPTG addition. The values represent the means±SD for at least triplicate independent experiments.