Table 1. Oligonucleotide primers used in this study.

Name	Sequence
A-up	5′-GGAATTCCATATGGGGCAATCACACACGC-3′
P14K-down	5′-CCGGAATTCTCAAGCCATTGCGGCAACGA-3′
B-down(rbs)	5′-CATATCTATATCTCCTTTCACGCTGGCTCCAGGTAGTC-3′
P14K-up(rbs)	5′-TGAAAGGAGATATAGATATGAAAGACGAACGGTTTC-3′
B-down(Strep)	5′-GCGGGTGGCTCCAGCTTGCCATATCTATATCTCCTTTCACGCTGGCTCCAGGTAGTCATC-3′
P14K-up(Strep)	5′-AAGCTGGAGCCACCCGCAGTTCGAAAAGGGTGCAAAAGACGAACGGTTTCCATT-3′
HisT7A-up	5′-GGAATTCCATATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCCGCGCGGCAGCCATATGGCTAGCATGACTGGTGGACAGCAAATGGGTCGCGGGCAATCACACACGCATGACCAC-3′
B-up	5′-GGAATTCCATATGAATGGCATTCACGATACT-3′
B-down(BA)	5′-CATATCTATATCTCCTTTCACGCTGGCTCCAGGTAGTC-3′
A-up(BA)	5′-TGAAAGGAGATATAGATATGGGGCAATCACACACGC-3′
A-down(AP)	5′-CATATCTATATCTCCTTTTAATGAGATGGGGTGGGTT-3′
P14K-up(AP)	5′-TAAAAGGAGATATAGATATGAAAGACGAACGGTTTC-3′

Italicized letters denote the NdeI (in A-up, HisT7A-up, and B-up) and EcoRI (in P14K-down) restriction sites, respectively; bold letters indicate the overlapping nucleotides; underlined letters indicate the His-Tag and T7-Tag, respectively.