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. 2012 Nov 30;7(11):e50645. doi: 10.1371/journal.pone.0050645

Figure 6. The histone acetyltransferase p300 acetylates full length YY1 wild type in vitro.

Figure 6

(A) Cold in vitro acetylation assay reactions using purified p300-HAT domain and purified GST-YY1 (left panel) and non-tagged YY1 (right panel) as substrate. The reactions were performed at 30°C for 30 minutes followed by Western blot. The blot was probed with anti-acetyl-lysine antibody, then stripped and reprobed with anti-YY1 antibody. (B) Cold in vitro acetylation assay reactions using purified p300-HAT domain and purified GST-YY1 (1–200 a.a.) wild type, S180,184A and S180,184D deletion mutants. The reactions were performed at 30°C for 1 hour, followed by Western blot. The blot was probed with anti-acetyl-lysine antibody, then stripped and reprobed with anti-YY1 antibody.