Table 1. Summary of the materials and results of pyrosequencing.

Category	Library 1	Library 2	Library 3-1	Library 3-2	Total
RNA source	TotalRNA	mRNA	mRNA	mRNA
cDNA amplification	NuGEN	NuGEN	Roche Std.	Roche Std.
Run regions	1/8	1/8	1/8	1/8
Valid reads	76 892	110 009	122 436	133 578	442 915
Valid reads(minus rRNA)	32 093	93 009	56 899	65 360	247 361
Number ofcontigs					17 328
Number ofsingletons					31 921
Average sizeof contigs					549