Figure 1. 15dPGJ2 reduced NF-κB p65 activity in amniocytes and myocytes.

Protein was extracted from IL-1β stimulated and 15dPGJ2 treated cells and levels of nuclear p65 were examined using immunoblotting. A dose response of 0.1–32 µM of 15dPGJ2 was used (n = 3). Representative immunoblots are shown for amniocytes, (A), myocytes (B). Immunoblots were re-probed for β-actin as an internal loading control.