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. 2012 Nov 30;7(11):e50734. doi: 10.1371/journal.pone.0050734

Table 2. The incubation conditions and the catalogue numbers for all antibodies used.

Primary antibody Secondary antibody Cat number
P-p65 (Ser536) 1∶1000 Overnight at 4°C Anti-rabbit; 1∶2000 1 hr RT Cat 3033s
p65 1∶1000 Overnight at 4°C Anti-mouse; 1∶200 1 hr RT Cat no 8008
CRTH2 1∶200 Overnight at 4°C Anti-goat: 1∶2000 1 hr RT Cat no. 23092
CRTH2 1∶500 Overnight at 4°C Anti-rat; 1∶3000 1 hr RT Cat no. 2178
CRTH2 1∶500 Overnight at 4°C Anti-rabbit; 1∶2000 1 hr RT Cat no. PR-4029
β-actin 160∶000 30 minutes at RT Anti-mouse; 20∶1000 15 mins RT Cat no. A 2228