Table 2. The incubation conditions and the catalogue numbers for all antibodies used.
Primary antibody | Secondary antibody | Cat number | |
P-p65 (Ser536) | 1∶1000 Overnight at 4°C | Anti-rabbit; 1∶2000 1 hr RT | Cat 3033s |
p65 | 1∶1000 Overnight at 4°C | Anti-mouse; 1∶200 1 hr RT | Cat no 8008 |
CRTH2 | 1∶200 Overnight at 4°C | Anti-goat: 1∶2000 1 hr RT | Cat no. 23092 |
CRTH2 | 1∶500 Overnight at 4°C | Anti-rat; 1∶3000 1 hr RT | Cat no. 2178 |
CRTH2 | 1∶500 Overnight at 4°C | Anti-rabbit; 1∶2000 1 hr RT | Cat no. PR-4029 |
β-actin | 160∶000 30 minutes at RT | Anti-mouse; 20∶1000 15 mins RT | Cat no. A 2228 |