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. 2012 Nov 30;7(11):e50637. doi: 10.1371/journal.pone.0050637

Figure 8. GATA-4 enhances FOG-2 SUMOylation.

Figure 8

(A) COS-7 cells were transfected with constructs containing FOG-2, GFP-SUMO-1 and GATA-4 as indicated in the figure. Cells were boiled directly in Laemmli buffer, run for Western blotting and probed with the indicated antibodies. (B) The increase in FOG-2 SUMOylation was quantitated by densitometry using ImageQuant TL 1D, version 7.0 (GE Healthcare). The graph shows the ratio of total SUMOylated FOG-2 to total FOG-2 (percentage). Asterisks indicate non-specific bands detected by the FOG-2 antibody. IB, immunoblot.