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. 2012 Nov 30;7(11):e50730. doi: 10.1371/journal.pone.0050730

Figure 6. Average quantity of p53 per cell following chemical transfection with 40 µg/mL F127-6G6 E6 antibody.

Figure 6

p53 quantity is evidenced by intensity of p53 staining. Cells transfected with the antibody show a higher quantity of p53 per cell (i.e. a higher intensity of p53 staining) after 48 hours than those in control wells treated with only the HiPerFect (HiP) transfection reagent or culture medium (Med) alone (P = 0.005, P<0.001, n = 3 for both). Also, SiHa cells showed a greater quantity of p53 per cell than CaSki (P<0.001, n = 3). Statistical analysis was performed using a two-way ANOVA followed by Tukey HSD contrast tests post hoc. Error bars represent mean +/− SEM. “*” denotes significance.