Figure 4. HSC chimerism.

(A) The chimerism of LT-HSC, ST-HSC/MPP and MP in the recipient mice BM in competitive transplantation assay. The recipient mice (CD45.1⁺) were lethally irradiated and transplanted with a mixture of 1×10⁶ test (Tak1^Het and Tak1^iKO, CD45.2⁺) and 2×10⁵ competitor wild type (CD45.1⁺) BMN cells. Three weeks after tamoxifen injection, these recipients were analyzed for the chimerism of LT-HSC (CD34(–), LSK), ST-LSK/MPP (CD34(+) LSK) and MP (LK). Gating strategy and one result for each of the Tak1^Het and Tak1^iKO test BMN transplanted animals are shown. (B) Gating strategy for SLAM-LSK and one result for each of the Tak1^Het and Tak1^iKO test BMN transplanted animals are shown. (C) The chimerism of LT-HSC (CD34(–), LSK), ST-LSK/MPP (CD34(+) LSK), MP (LK) (n = 4) and SLAM-LSK (n = 3) in the recipient mice BM. The chimerism in each recipient is plotted, and the bars represent the average. * p<0.05 [Tak1^Het versus Tak1^iKO or Tab1Tab2^iDKO].