Table 1. Bacterial strains and plasmids used in this study.

	Relevant characteristicsa			Reference or source
X. campestris pv. vesicatoria
85-10	Pepper-race 2; wild type; Rifr			[51], [73]
85-10ΔhrcQ	hrcQ deletion mutant of strain 85-10			This study
85-10ΔhrcQ::hrcQ	Derivative of strain 85-10ΔhrcQ carrying hrcQ-c-myc under control of the native promoter inserted into the hpaFG region			This study
85*	85-10 derivative containing the hrpG* mutation			[34]
85*ΔhrcQ	hrcQ deletion mutant of strain 85*			This study
85*ΔhrcQ::hrcQ	Derivative of strain 85*ΔhrcQ carrying hrcQ-c-myc under control of the native promoter inserted into the hpaFG region			This study
E. coli
BL21 (DE3)	F- ompT hsdSB (rB - mB -) gal dcm (DE3)			Stratagene, Heidelberg, Germany
Top10	F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu) 7697 galU galK rpsL (StrR) endA1 nupG			Invitrogen, Karlsruhe, Germany
DH5α	F- recA hsdR17(rk-,mk+) Φ80dlacZ ΔM15			Bethesda Research Laboratories, Bethesda, Md.
DH5α λpir	F- recA hsdR17(rk-,mk+) Φ80dlacZ ΔM15 [λpir]α			[74]
Plasmids
pBlueskript(II) KS	Phagemid, pUC derivative; Apr			Stratagene
pBRM	Golden Gate-compatible derivative of pBBR1MCS-5 for lacpromoter-driven gene expression; contains a 3× c-Mycepitope-encoding sequence			[75]
pBRM-P	Derivative of pBRM without lac promoter			This study
pBRMhrcN	pBRM derivative encoding HrcN-c-Myc			[75]
pBRMhrcQ	pBRM derivative encoding HrcQ-c-Myc			This study
pBRMhrcQStop	pBRM derivative encoding HrcQ			This study
pBRM-PhrcQ	pBRM-P derivative containing hrcQ-c-myc and 299 bp upstream region			This study
pBRM-PhrcQStop	pBRM-P derivative containing hrcQ and 299 bp upstream region			This study
pBRMhrcV	pBRM derivative encoding HrcV-c-Myc			N. Hartmann and D. Büttner, unpublished
pBRMhrcV324-645	pBRM derivative encoding HrcV324-645-c-Myc			N. Hartmann and D. Büttner, unpublished
pBRMxopJ	pBRM derivative encoding XopJ-c-Myc			[26]
pDGW4MhpaA	Derivative of pDGW4M encoding HpaA-c-Myc			[80]
pDMhrcL	Derivative of pDSK602 encoding HrcL-c-Myc			[20]
pGEX-2TKM	GST expression vector; ptac GST lacI q pBR322 ori; Apr, derivative ofpGEX-2TK with polylinker of pDSK604			Stratagene[38]
pGhpaA	pGEX-2TKM derivative encoding GST-HpaA			[76]
pGhpaB	pGEX-2TKM derivative encoding GST-HpaB			[18]
pGhpaC	pGEX-2TKM derivative encoding GST-HpaC			[18]
pGhrpB2	pGEX-2TKM derivative encoding GST-HrpB2			[62]
pGhrpE	pGEX-2TKM derivative encoding GST-HrpE			[55]
pGhrcL	pGEX-2TKM derivative encoding GST-HrcL			[20]
pGhrcN	pGEX-2TKM derivative encoding GST-HrcN			[76]
pGhrcQ	pGEX-2TKM derivative encoding GST-HrcQ			This study
pGhrcU	pGEX-2TKM derivative encoding GST-HrcU			[21]
pGhrcU255-357	pGEX-2TKM derivative encoding GST-HrcU255-357			[21]
pGxopA	pGEX-2TKM derivative encoding GST-XopA			[17]
pGxopJ	pGEX-2TKM derivative encoding GST-XopJ			This study
pGxopF1	pGEX-2TKM derivative encoding GST-XopF1			[18]
pG300	pGEX-2TKM derivative encoding GST-AvrBs3			[77]
pG356F	pGEX-2TKM derivative encoding GST-AvrBs3Δ2 which lacks theN-terminal 152 amino acids			[17]
pGavrBs350	pGEX-2TKM derivative encoding GST-AvrBs31-50			[17]
pLAND-P	Derivative of pOK1 carrying fragments of the hpaFG region flanking lacZ, the lac promoter and a triple c-Myc epitope-encoding sequence			This study
pLAND-PhrcQ	Derivative of pLAND-P carrying hrcQ and 299 bp upstream region			This study
pOK1	Suicide vector; sacB sacQ mobRK2 oriR6K; Smr			[78]
pOKΔhrcQ	Derivative of pOK1 carrying the flanking regions of hrcQ			This study
pRK2013	ColE1 replicon, TraRK+ Mob+; Kmr			[49]
pUC119	ColE1 replicon; Apr			[79]

^aAp, ampicillin; Km, kanamycin; Rif, rifampicin; Sm, spectinomycin; Gm, gentamycin; r, resistant.